Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDQKETIMQTFSPKAVIERLQNIVGRSYVLTDDQSTRQYRQGRR-FGEGKVLAVVVPGTLLEQWQVLQAAIEAGCIVIMQAANTGLTGGSTPYGDDYDRPVLVMSTRRLKGIQVIHD-GKQVICLPGATLDNLEQILKGYDREPHSVIGSSCIGASVLGGVCNNSGGALV-RRGPAYTELALYAQVNAAGQLELVNHLGVNLGSTPEEILTRLEKQQYQAVDILDDNEKQASDHRYGHDVTQVDEDTPARFNADPSRLFEASGSAGKVCVFA-VRLDTYEKVE-SSVFYIGSNDADDLTAIRRYLLTSLPSLPIAGEYIHRDAYLIGEKYGKDTFLFIEKFGTANVPKAFAMKDKVDGFLEKFKIKGLTDQILQAITFFLPSHLPKRMTEYRDRYEHHLVLRVENNSKAQTEQFLKEYFTVH-----QSGNYFVCSEEEGRKAFLHRFAIAGAAIRYRDTHRSEVEDIVALDIALRRNDREWVEQLPAEME--KKIIH-------KLYYGHFFCHVFHQDYILK---K--G-H-DPLEIEHQMWKLLDARRAEYPAEHNVGHLYIAKPALANFY------------QKLDPTNSFNVGIGHTSKLKYWGKAKS 3PM9 Chain:B ((12-476)) -----------TLSPELIARFTAIVGDKHALTDPLELEAYITEERNLYRGHSPLVLRPGSTEEVVAICKLANEARVALVPQGGNTGLVGGQTPHN-----GEVVISLKRMDKIREIDTSSNTITVEAGAILQRVQEKAAEVDRLFPLSLGAQG-SCTIGGNLSTNAGGTAALAYGLARDMALGVEVVLADGRVMNLLSKL----------K-------------------K----------------DNT--GYDLRDLF--IGAEGTLGIITAATLKLFPKPRAVETAFVGLQSPDDALKLLGIAQGEAAGNLTSFELIAETPLDFSVRHANNR-------------------------------------------DPLE-ARY----------PWYVLIELSSPRD-DARAALESILERGFEDGIVVDAAIANSVQQQQAFWKLREEISPAQKPE----GGSIK----HDISVP------VAAVPQFIEQANAAVVALIPGARPVPFGHLGDGNIHYNVSQPVGADKAEFLARWHDVSQVVFEVVLRLGGSISAEHGIGV-MKR-DELAEVKDKTAIELMRSIKALLDPHGIMNPGKVV------------

General information: TITO was launched using: RESULT: Template: 3PM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2463 18310 7.43 42.88 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 7.43 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_3PM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PM9-query.scw PDB file : Tito_Scwrl_3PM9.pdb: