Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLVSRIQNLPIGKFHYTLLWVVGLGWMFDAMDTGIIAFIMTTLVKDWSLTPVESGWIVSIGFVGMAIGAVCSGALADRFGRKTVFAMTMAIYSIATALCAFAPDLKWLLIFRFIVGLGLGGQLPVAVTLVSEYVPAHVRGRFIVLLESFWGLGWLVAALISYFIIPKFGWHIAFLIGGLPLIYIL-VIWKKVPESIPYLINRGRIEEAHALVQKLEAEACVQIVHHIEVVPVAMRQKVSFKQLWSGQFARRSLMLWLIWFGIVYSYYGIFTWLPSLLVK-QGYDIVKSIEYVLLMILAQLPGYLAAAWFVERLGRKITLAAFIGFCALSAYFFGQ-----ADSVNSIMFWGCLMSFFNLGAWGVLYTYTPEQYPANIRAFGSGWASAMGRIGGIVAPMVVTHMMVAKNGFNHVFMMFTVVLLAVALVILILGEETQGKRLESIGL 4ZP0 Chain:A ((5-366)) -------------RQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIRESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEK---LSL------------K------------EL-GRDYKLVLKNGRFVA---GALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLV-RLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLN---------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1730 -301528 -174.29 -849.37 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -174.29 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4ZP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZP0-query.scw PDB file : Tito_Scwrl_4ZP0.pdb: