Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVEKLRRKIMQSKTLFLLGLCTIFSTTVSALPLDSKGAKQRLSQAAKQKVFQGKIDLDALVSNDSNDLIVEYNIPSSSVSSGLERRSYIATNKKNLQTRFSRAGGVQVLRDYNNLPLAFYRISNREALIALLNDPNVKAVYPNRINRTTTTESLPLINQPQANTNG-FTGEGSSVAVLDTGVNYLHSDFGCTAVNTPSSTCRVVYSFDSAPDDGTLDDDGHGSNVSAIVSK---------VATKTKIIGIDVFRKVRSQGKWVSTAYDSDILAGINWAVNNAQTYN----------------IKAVNLSLGVPGVKYTSECSDSSYGTAFANARAAGVVPVVASGNDAFPDGISSPACVAGAVRVGAVYDSNIGGVSWGNPVKCSDPTTAADKVACFSNGGSLVTLLAPGAMITAGG-----YTMGGTSQATPHVAGAIALLRANSVSPTESIDQTISRLKATGKPITDSRTGLVFPR-------------IDLLAATNGLTVN 2E1P Chain:A ((4-398)) ------------NTIRVIVSV-------------------------------DKAKFNPHEVLGIGGHIVYQFKLI----------PAVVVDVPANAVGKLKKMPGVEKVEFDHQAVLLGKPSWLGG---------------GSTQPAQTIPWGIERVKAPSVWSITDGSVSVIQVAVLDTGVDYDHPDLAANIAWCVS----TLRGKVSTKLRDCADQNGHGTHVIGTIAALNNDIGVVGVAPGVQIYSVRVLDAR-------GSGSYSDIAIGIEQAILGPDGVADKDGDGIIAGDPDDDAAEVISMSLGGPA-------DDSYLYDMIIQAYNAGIVIVAASGNEG-APSPSYPAAYPEVIAVGAIDSN--------------------DNIASFSNRQ--PEVSAPGVDILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPVGTFDDISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG-

General information: TITO was launched using: RESULT: Template: 2E1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2448 -90495 -36.97 -257.82 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -36.97 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_2E1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E1P-query.scw PDB file : Tito_Scwrl_2E1P.pdb: