Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFLQRSNQHSNNNRLKRMKSHLRVHYFQHIAGEGFGSCYSFLKAHHATITATEFFALPVDLPLEIEALPYIEDVDLLLIMGGTMSVNDEANYPWLKIEKRWIRRYLAAGKPAIGLCLGGQLIANALGAAVSRNRYQELGWSTVQRVPNLPKESFL--LPE--------KINVMQWHSETF-EIPKGAIHLAENSVCRNQMYQIG-SNVLGFQFHPEMTP-----KVLNLLLENEQELSIFKGEYVQSLDELHHCDIQKFEQGNQLLNRAIEFVVNQ 1GPM Chain:A ((4-223)) -------------------NIHKHRILILDFGS-QYTQLVARRVRELG--VYCELW--AWD---VTEAQI-RDFNPSGIILSGGPESTTE-ENSP------RAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEASNEREFGYAQVEVVND---SALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDI------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -95834 -96.70 -529.47 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -96.70 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_1GPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPM-query.scw PDB file : Tito_Scwrl_1GPM.pdb: