Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLRLDRLHYCILMSMGCISSPLVWAEDLNSDVAKLPTLHVEA------------TRTDTGYLQTPASVFRIEAPQVDSSSQVNLTEVVKGIPSLQIRNRENYAQDLQLSMRGFGARSTFGVRGIRLYVDGIPATMPDGQGQTSNIDLSSLDHVEVLTGPFSSLYG-NSSGGTILTSTKEGQGKDSIELSYSGGSHDKSRAGLVLQGGAKGANEPSYIISSSYFDTDGYRE---HSGAEKVLNNAKLSWNLDDGSKINWVTNYVKINADDPGGLTRADWQ---NNPKQ---VV-QNVLDY--NARKEIEQTQTGLTWSKPINDQHELYAMTYMGQRQVTQYQSIPDTVQK--NPNTPYQA-G-GVIDFKRNYYGADFRWTGKELLPNTTLSIGVALDAMKEDRQGYQNFNDT---------------------GDKGV--KG---ALRRDEDNTLWNIDPYVQASWQFLPTWRLDTGVRYSNVHYKSKDYYIVGL---NGDNSGKTSYEEVLPSVALSWQITPEVLAYASYAKGFETPTFTEMAYPAQGGASTLDLKPSTSDTYETGLKSQ-N-QLGDFTLAVFQTKTKNDIVSAESFGGRSTFRNADKTLREGVEFAWNKKLWRDLIAIASYTYLDATFDSTVPAAGKISEIPEGNAIPGIAKNQAYVSLAWQPSHGLYGGVDVQYMDKVYVNDTNSDAAPSYSVTSANVGYAWVMGDWKVNSFARVDNLFDRNYAGSVIVNDSTQPVGRYFEPADGRNWSAGLRVIKQF 5FOK Chain:A ((28-721)) --------------------------------------------DETTYNVDRSASKKYTAPLLDTPKTVTVIPQQVIKDTGALTLADALRTTPGITFGA-------DRPFIRGFNAE-------SDTFLDGMRDVAS------QTREVFNVEQIEVSKGPGSAYTGAGSTGGSLNLISKTAKQDNFTDAGFTWGSDQTRRTTLDVNRMI--GDNAAFRLNLMKH--DAHVAGRDEVSVSRWGVAPTVTFGFDTPTRATLSYYHLSTDDMPDYGLPLTNVNRSKANPSKPASVDRDNFYGLKDRDYRKSTTDSGTFRIEHDLNDNLTLSNSTRLVRTTLDYIVSNPDDSRGNVANGYVYRSAKSRNSTSKGWVNQTDLKANFETGFIKHTLVTGLEFSYEDVHNRPYAITSGGGAGNTCNARLLASGDCTSLNRPTPGDNWTGSITDGLAYTDTDTKTSAAYVFDTLKLSEQWELNLGLRYDDFDTKSSGYQTAGRNGPAGYFKRENNSHFWNYQTGLVYKPAPNGSIYLAWSTSSN-P---------------------RNRNLELGTKWAFFDDALSLNAALFRTDKTNAR----------LQVLDGEQRVQGVELGFNGKLTEKWKVFGGYTYLDSEIRKST------VKSDEGNKMPQTAQNNFTLWTTYDLLQNFTIGGGTTYVDKQYGNTANSTYIPSYWRYDAMASYKVS---KNVDLQLNVQNLTDKRYFDQVYS-------THMAHVAPGRTALLGVNFHFSA

General information: TITO was launched using: RESULT: Template: 5FOK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3694 202535 54.83 339.82 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 54.83 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_5FOK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FOK-query.scw PDB file : Tito_Scwrl_5FOK.pdb: