Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATGTVKLHRVFKAPAERVYRAFLDPDALVKWMAPHGFTAKVHSFDAKVGGSYKMSFTNFSTGSTHSFGGTYVELVPNELIRYNDQFDDAYL----PGTMQVTIT-KTVLVGTEIHITQEGIPEV----IPVEACYLGWQESLSLLKLLVEADIPDQ 1XFS Chain:A ((8-169)) -AELDLMLKRELAVPVNLVWRGLTEPELLKKWFVPKPWSISDCRVDLRPGGEFYTVMQDP-EGNKFPNSGCFLEVTDEKRLIWTSALVKNYRPAVP--VMTAVIELQPTSSGTRYTACAMHNTPGQRKLHEEMGFHEGWGTTITQLEELLKQEKA--

General information: TITO was launched using: RESULT: Template: 1XFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 615 -36084 -58.67 -254.11 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -58.67 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1XFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XFS-query.scw PDB file : Tito_Scwrl_1XFS.pdb: