Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYKALLSSLSIALFSLSLAGCNDNDSQETAVSTPKQPNILFIMADDLGYSDLGAFGG-EIHTPNIDSLAQEGRILTDYHT-APTCSPTRSQLISGTDHHLAGIGAMAELTPDHLKGQPGYEGYLNERSLSITQVLKDNGYRTYISGKWHLGLTPE------TNAHAKGFDHSFTLLQGLD--------HHFKQAPSA--FK----RNS----------------TY---------------TEDGQII-----------P--VSALP-DDFFSTNYFTDKLLSYLESGKNSGKPFFAYAAYTAPHWPIQAPAEYREKYRGAYDVGYDAIRNARIERQKQLGIIPVNFEAAEPIATQNAPQKYGKYELTAEQKALEARKMEIYAGMVENLDANVGRIIQYLKQNNLYDNTLIFFVSDNGAEGFVRGSYGCESGFDNSVANVGTPTSYHYIGPRWAEVSAAPFHLWKDTAGEGATTAPAIVKLPNQKKAEETNHSFASVLDVFPTLLDYAHIPVPQGQYKGRTINTPSGYSWKSVLENKSQT-IRPANFSFADELHGS------KYAKQGEWKIALQGRPE-----------------LGTGSWELYNIATDRGENHNVAQL----YPAKVQELLAVYQKYTEQNGVQEYNAK 1P49 Chain:A ((2-521)) ---------------------------------AASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPL-TPSRAAFMTGRYPVRSGMASWSRTGVFL---FTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQR--NTETPFLLVLSYLHVHTALFSSKDFAGK-----------------------------------------------------------SQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKG-----------------------EIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPED-------RIIDGRDLMPLLEGKSQRSDHE--FLFHYCNAYLNAVRWHPQNSTSIWKAFFFTPNFNPVCFATHVCFCFGSYVTHHDPPLLFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLP-------

General information: TITO was launched using: RESULT: Template: 1P49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2438 24296 9.97 57.85 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.97 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_1P49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P49-query.scw PDB file : Tito_Scwrl_1P49.pdb: