Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLAQLVREEMLHYEQVLEFMTKRGQEWKGLS----AGRYAGGLRKEIRTYEPEALIDVLVIGAFVEARSCERFYALAPCV-DDELGRYYRYLLKSESRHFEDYLALALDVAKTAKMKDPEEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA 2FZF Chain:B ((27-161)) --------------------------------------MAIKAEIGAREFYKSLAEKIK-IEALKEKINWLAEEEKKHEALLRKLYSQMFPGKEVVFPKEHIGPELQPVARELEK--VQDIIDLIRWAMKAEEIAAEFYLKLEEMVKEEEKKRLMRYLADMERGHYYTLRAEYELL-------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FZF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 440 -8061 -18.32 -62.00 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -18.32 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_2FZF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZF-query.scw PDB file : Tito_Scwrl_2FZF.pdb: