Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLMIDNYDSFTYNIVQYFGELNQEVKVVRNDQVTLEDIERWQPKYLVIGPGPCSPSEVGISIPAINHFA-GKIPLFGVCLGHQSIGQAFGGKIVRAKTVMHGRLSDMYHS-NKGIFSNLPSPFSATRYHSLVIDQETLPDCLEVTCWTNEADGSMEEIMGVKHKTLPVEGVQFHPESILSQHGHQIFKNFLDIYA 1WL8 Chain:A ((2-186)) MIVIMDNGGQYVHRIWRTLRYLGVETKIIPNTTPLE-EIKAMNPKGIIFSGGPSLENT-GNCEKVLEHYDEFNVPILGICLGHQLIAKFFGGKVGRGEKAEYSLV-EIEIIDE--IFKGLPKRLKVWESHMDEVK--ELPPKFKILARSET-----CPIEAMKHEELPIYGVQFHPEVAHTEKGEEILRNFAKLC-

General information: TITO was launched using: RESULT: Template: 1WL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1068 -32222 -30.17 -178.02 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -30.17 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_1WL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WL8-query.scw PDB file : Tito_Scwrl_1WL8.pdb: