Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNMTFIVAVQLKDSIVVAVDNKYLTLKDKEKD---HFEEHFSSKLYAWHSGI----ITGTGEHYVIDKAVR----LFINSADADIEKLPLCLNISRQIREMEVGQHEQIQSSK------LLYSQYSENGAKLFAIEPTEETGKYQRTEFKENDLIIWLF-NPNIQSIS--GNLKTLYSNPR----------PKVSFDRIEDWLDYYISAIAEIYEKQSCIDSWMSGSFDIFFQTEGEYFYKHIQNNPTVHLE-------------------------------------- 3E4R Chain:A ((25-315)) AAEPAQLRIGYQKAVSSLVLAKQHR-LLEQRFPRTKITWVEFPAGPQLLEALNVGSIDLGGAGDIPPLFAQAAGADLLYVGWVPPTPKAETILVPSKSALRTVADLKGKRIAFQKGSSAHNLLLRVLAKSGLSMRDITPLYLSPANARAAFAAGQVDAWAIWDPWYSALTLDGSARLLANGEGLGLTGGFFLSSRRYATAWGPFVQQVMGTLNQADGLLERDRAGSIKTLAQVSGLPPAVVERTLAHRPPASVQPLSAQVIKAQQATADLFYAQRLLPKRVLVAPAVWRAPD

General information: TITO was launched using: RESULT: Template: 3E4R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1206 6759 5.60 30.58 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 5.60 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_3E4R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E4R-query.scw PDB file : Tito_Scwrl_3E4R.pdb: