Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNIDLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQN-E-EEVCRIVASHTFAVYLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPL---ETSGVQRYSLMEDQLVIAGDPK--------------SKLWLVREANSGVFHYTQRYFEEMNIQDK-KMIVKSNDVIVALLKEGIGRSILSKRSVPE-----NVPMTPL---NFHRYFYFIQR-TNLVSDQLLEIAEEILRYNESTETSHSEENLSW 2ESN Chain:B ((9-305)) DLNLLLVFDALYRHRNVGTAASELAISASAFSHALGRLRQGLDDELFLRQGNR-MQPTQRAEHLAAAVAAALRALGEGLEEWRPFVPGQSQRTFVFAATDYTAFALLPPLMNRLQHSAPGVRLRLVNAERKLSVEALASGRIDFALGYDEEHERLPEGIQAHDWFADRYVVVARRDHPRLAGAPTLEGYLAERHAVVTPWNEDSGVIDRLLARSGLRREVAVQLPTVLAALFLAGSTDFLLTAPRHAARALAEAAGLALYPAPFDIPPYVLRLYSHVQ----DAHAWMIGQLKGLDIS------------

General information: TITO was launched using: RESULT: Template: 2ESN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1078 -34496 -32.00 -130.67 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -32.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_2ESN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ESN-query.scw PDB file : Tito_Scwrl_2ESN.pdb: