Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQSISVQDFNKLPL--DADSIVLDTRN-TLDYTKNHLSHSLSIPAAILPRQLKNLDPKKLYYILSYTGRRS--EALADQLTANGFRAVYIIGGMEALQSSVA 3FLH Chain:B ((15-114)) --LYIDHHTVLADMQNATGKYVVLDVRNAPAQVKKDQIKGAIAMPAKDLATRIGELDPAKTYVVYDWTGGTTLGKTALLVLLSAGFEAYELAGALEGWKGMQ-

General information: TITO was launched using: RESULT: Template: 3FLH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 426 -6923 -16.25 -72.87 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -16.25 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.796

(partial model without unconserved sides chains): PDB file : Tito_3FLH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FLH-query.scw PDB file : Tito_Scwrl_3FLH.pdb: