Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVITIRPTDTEPFT----NDLSMLLIEKNIRQLGFT---------------GFIAVNLFSYTKAKS-PRVFLRGNDEQSLEILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLSHPLSIHVRNEWQLADSQLLFQEK 1MUG Chain:A ((10-112)) --------------------------------------LRVVFCGINPGLSSAGTGFPFAHPANR-FWKVIYQAGFTDRQLKPQEAQHLLDYRCGVTKLVD-RPTVQANEVSKQEL-HAGGRKLIEKIE-DYQPQALAILGKQAY----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1MUG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 294 1643 5.59 19.79 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 5.59 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1MUG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MUG-query.scw PDB file : Tito_Scwrl_1MUG.pdb: