Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QVFG-----CFVQMSVRTQD---SKDETNIS------------SGGYLLTTNPGQLVFTDGNNNITVKVDKDCQSAGISYHRSEVHFTALQKLSENGGPFEFDQPLIELQ 5J4F Chain:A ((22-119)) EVVHFLEGVCFEKLHIEVLNENSSHKEIRICMPKGAVMDKHKAPGAISVQVLEGKIVFEVGDEKIEMPKGALISLEAQVLHRLDALENSVIRLSLSKK------------

General information: TITO was launched using: RESULT: Template: 5J4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 225 1072 4.76 13.74 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 4.76 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_5J4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J4F-query.scw PDB file : Tito_Scwrl_5J4F.pdb: