Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FCEYYIKTPEGWQLLGWVKIGGQDELTW-KGDSIGV--KAEDTNCKVVLVNGQR--APV-WLTAVP- 1S1N Chain:A ((153-212)) -------TGEEYIAVGDFTAQQVGDLTFKKGEILLVIEKKPDGWWIAKDAKGNEGLVPRTYLEPYSE

General information: TITO was launched using: RESULT: Template: 1S1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 202 -2860 -14.16 -53.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -14.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_1S1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S1N-query.scw PDB file : Tito_Scwrl_1S1N.pdb: