Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSG-------PTYRGAVPWYTINLDLPPYKRWHELMLDKAP------VLKVIVNSLKNMINTFVPSGKIMQVVDEKLPG------LLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNE-RFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKT------KILAPAYFILGGNQSGEGC---VITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 1CJD Chain:A ((15-383)) LRNQQAMAANLQARQI---VLQQSYPVIQQVETQTFDPANRSVFDVTPANVGIVKGFLVKVTAAITNNHATEAVALTDFGPANLVQRVIYYDPDNQRHTETS-GWHLHFVNTAKQGAPFLSSMVTDSPIKYGDVMNVIDAPATIAAGATGELTMYYWVPLAYSE---TDLTGAVLANVPQS---------------------KQRLKLEFA-NNNTAFAAVGANPLEAIYQG--AGAADCEFEEISYTVYQSYLDQLPVGQNGYILPLIDLSTLYNLENSAQAGLTPNVDFVVQYANLYRYLSTIAVFDNGGSFNAGTDINYLSQRTANFSDTRKLDPKTWAAQTRRRIATDFPKGVYYCDNRDKPIYTLQY---GNVGFVVNPKTVNQNARLLMGYEYFTSR---------------------

General information: TITO was launched using: RESULT: Template: 1CJD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 3873 2.28 11.39 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 2.28 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_1CJD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CJD-query.scw PDB file : Tito_Scwrl_1CJD.pdb: