Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLST-DDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVH----HSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGF-VEVIHD--KKQYQISELCTQFCWPFNVKVAVRDLSIKD-DILAAT-----------PGLQLEEDI-TDSYLLISDFA-------NPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL 1C3J Chain:A ((1-351)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKIAIINMGNNVINFKTVPSSETIYLFKVISEMGLNVDIISLKNGVYTKSFDEVDVNDYDRLIVVNSN----LAILSAQKFMAK-----YKSKIYYLFTDIRLPFSQSAYLY-TEEELLIKSPIKVISQGINLDIAKAAHKKVDNVIEFEYFPIEQYKIHMNDFQLSKPTKKTLDVIYGGSFRSGQRESKMVEFLFDTGLNIEFFGNAREKQFKNPKYPWTKAPVFTGKIPMNMVSEKNSQAIAALIIGDKNYNDNFITLRVWETMASDAVMLIDEEFDTKHRIINDARFYVNNRAELIDRVNELKHSDVLRKEMLSIQHDILNKTRAKKAEWQDAFKKAIDL----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1C3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1560 31321 20.08 102.69 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 20.08 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_1C3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1C3J-query.scw PDB file : Tito_Scwrl_1C3J.pdb: