Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEV----------PKQNGKYETGQLFLHSIFG----YRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKT------HTYYQVLIDARDCPHISQR-------------SQTEAVTFLANHDDSRALYAIPG---LDYVSHEDILPYTSTDQVPIQHELFERFLLY------DQTKAPPFVARETLRAWQEKNHPWLELSDVHRE-TTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVS----LQASSGHMWGTFRF-ERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW---------- 4D10 Chain:C ((3-403)) -----------SALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMG----LVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQ----------LSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQF

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1456 16086 11.05 46.90 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : 11.05 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: