Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVEKEEKGEEMKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPDLMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSIVQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNELI 5CLJ Chain:B ((23-143)) -------------PVVGIIMGSQSDWETMRHADALLTELEIPHETLIVSANRTPDRLADYARTAAERGLNVIIAGAGGAAHLPGMCAAWTRLPVLGVPVESRALKGMDSLLSIVQMPGGVPVGTLAIGASGAKN---------------------------------------

General information: TITO was launched using: RESULT: Template: 5CLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 668 -85258 -127.63 -704.61 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -127.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_5CLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CLJ-query.scw PDB file : Tito_Scwrl_5CLJ.pdb: