Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIVISAYNEE-KYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTT---------------AIIQQFIKEDTEFNSIRLYNN--PKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQ--GEFVCGGPRPTIVEGKGK--WA--ETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNEQLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQFKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTIIYLDKISQINKNML 4P02 Chain:A ((136-395)) ---------------------------------QPEELPTVDILVPSYNEPADMLSVTLAAAKNMIYPARLRTVVLCDDGGTDQRCMSPDPELAQKAQERRRELQQLCR----ELGVVYSTRERNEHAKAGNMSAALERLKGELVVVFDADHVPSRDFLARTVGYFVEDPDLFLVQTPHFFINPDPIQRNLALGDRCPPENEMFYGKIHRGLDRW--GGAFFCGSAAVLRRRALDEAGGFAGET-ITEDAETALEIHSRGWKSLYIDRAMIAGLQPETFASFIQQRGRWATGMMQMLLLK-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4P02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1194 -6975 -5.84 -29.55 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -5.84 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_4P02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P02-query.scw PDB file : Tito_Scwrl_4P02.pdb: