Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MHLTHREVRDKLLSYSEGLQVYYELYQLLLFHF-------QEKNADHFFGLIEQELPTVHPLFQTVFWTFLRDRDKIINALKLPYSNAKLEATNNLIKVIKRKAFGFRNFNNFKK--RILMTLNIKKESTNFVLSRL 3JB9 Chain:S ((1-132)) MFCSISGETPKEPVISRVSGNVYEKRLIEQVIRETSKDPVTQQECTLEDLVPVKVPDFVRPRPPSAT-------SLPALLSLFQEEWDSVALEQFELRRNLTETKQELSTALYSLDAALRVISRLTKERDEAREALAKF

General information: TITO was launched using: RESULT: Template: 3JB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 226 8065 35.68 66.65 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain S : 0.63 3D Compatibility (PKB) : 35.68 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_3JB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JB9-query.scw PDB file : Tito_Scwrl_3JB9.pdb: