Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNYIRILAGDRVTVEMSPYDLTRGRITYRFK 2N78 Chain:A ((1-72)) MSKEDSFEMEGTVVDTLPNTMFRVELENGHVVTAHISGKMRKNYIRILTGDKVRVELTPYDLSKGRITYRAR

General information: TITO was launched using: RESULT: Template: 2N78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 269 -19602 -72.87 -272.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -72.87 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_2N78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N78-query.scw PDB file : Tito_Scwrl_2N78.pdb: