Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVL--DEYFTMMAVVSSDEK-QDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI 3OBI Chain:B ((6-88)) -QYVLTLSCPDRAGIVSAVSTFLFENGQNILDAQQYNDTESGHFFMRVVFNAAAKVIPLASLRTGFGVIAAKFTMGWHMRDRET-------

General information: TITO was launched using: RESULT: Template: 3OBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 315 -30742 -97.59 -384.27 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -97.59 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_3OBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OBI-query.scw PDB file : Tito_Scwrl_3OBI.pdb: