Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVEV-MGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPEQAGEFSFACGM---NMMHGKMIVE 5TK2 Chain:B ((13-100)) -------------------------------------AKVIEVELNDDYFNPNVITIPINESTTLLLKNKGK--SEHTFTIKKLGIDVVVESGKEKNITVKPKSAGTYELICRYHLLKGMEGKVIVK

General information: TITO was launched using: RESULT: Template: 5TK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 377 2493 6.61 29.67 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 6.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_5TK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TK2-query.scw PDB file : Tito_Scwrl_5TK2.pdb: