Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHCLMLSQNHLV----P--LENFQSGIIIHIEVE---DVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQGPAGLVLDFYRMK 3BQX Chain:A ((4-126)) QQVAVITLGIGDLEASARFYGEGFGWAPVFRNPEIIFYQMNGFVLATWLVQNLQEDVGVAVTSRPGSMALAHNVRAETEVAPLMERLVAAGGQLLRPADAPPHGGLRGYVADPDGHIWEIAFN-

General information: TITO was launched using: RESULT: Template: 3BQX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 412 -37303 -90.54 -327.21 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -90.54 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3BQX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BQX-query.scw PDB file : Tito_Scwrl_3BQX.pdb: