Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLKAKLEGKTSTKIETAALDADKVEEVIALIGSYKPEAVLNVALPYQDLTIMDACLATGVHYIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSGFDPGVTSVFSAYALKHYF----DEIHYIDILDCNGGDH-----GYPFATNFNPEINLREVSAPGSYWEDGKWVEVEAMSI----KREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSYLTHMKCLENVGLLRTDTINFNG----------------------------------------------------------QEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV-KDGVEKTIYIYNVCDHQECYAE-VGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE-LDPDPFMEALN-EYGLPWVVVENPQMVD 1FF9 Chain:A ((3-447)) TKSVLMLGSGFVTRPTLDVLTDSG---IKVTVACRTLESAKKLSAGVQ-----HSTPISLDVNDDAALDAEVA--KHDLVISLIP--FHATVIKSAIRQKKHVVTTSYVSP----------------------------AMMELDQAAKDAGITVMNEIGLDPGIDHLYAIKTIEE-VHAAGGKIKTFLSYCGGLPAPESSDNPLGYKFSWSSRGVLLALRNAASFYKDGKVTNVAGPELMATAKPYFIYPGF-AFVAYPNRDSTPYKERYQIPEADNIVRGTLRYQGFPQFIKVLVDIGFLSDEEQPFLKEAIPWKEATQKIVKASSASEQDIVSTIVSNATFESTEEQKRIVAGLKWLGIFSDKKITPRGNALDTLCATLEEKMQFEEGERDLVMLQHKFEIENKDGSRETRTSSLC-EYG-APIGSGGYSAMAKLVGVPCAVAVKFVLDGTISDRGVLAPMNSKINDPLMKELKEKYGIECKEK-------

General information: TITO was launched using: RESULT: Template: 1FF9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1759 -75276 -42.79 -204.55 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -42.79 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_1FF9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FF9-query.scw PDB file : Tito_Scwrl_1FF9.pdb: