Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVINKFVLMEQAKKVLKNAYCPYSKFPVGAAILFKDGKVITGANIENVSFGVTNCAERSAIFYGASQGYRKGDILAIAVAGE-TEDYLPPCNICRQVMVEFCEPDTLVFLLNGKGNILELRLEELVPYSFSSLEM 2FR5 Chain:B ((18-137)) --------LLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGYK--DFRAIAISSDLQEEFISPCGACRQVMREF-GTDWAVYMTKPDGTFVVRTVQELLPASF-----

General information: TITO was launched using: RESULT: Template: 2FR5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 619 -51992 -83.99 -436.91 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -83.99 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2FR5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FR5-query.scw PDB file : Tito_Scwrl_2FR5.pdb: