Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGA-RDGHDFIETAFENGAAVTLSEKEV-SNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRY----KTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAPADPIVEDYLPIDK------KVVRFGQG---AELEITDLVER-KDSLTFKANF--LEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQLAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK 4CVK Chain:A ((3-451)) EPLRLSQLTVALDARLI---GEDAVFSAVSTDSRAIGPGELFIALSGPRFDGHDYLAEVAAKGAVAALVEREVAAPLPQLLVRDTRAALGRLGALNRRKFTGPLAAMTGSSGKTTVKEMLASILRTQAGDAESVLATRGNLNNDLGVPLTLLQLAPQHRSAVIELGASRIGEIAYTVELTRPHVAIITNA-------FGGPEKIVEAKGEILEGLAADGTAVLNLDDK---AFDTWKARASGRPLLTFSLDRPQADFRAADLQRDARGCMGFRLQGVAGEAQVQLNLLGRHNVANALAAAAAAHALGVPLDGIVAGLQALQPVKGRAVAQLTASGLRVIDDSYNANPASMLAAIDILSGF----SGRTVLVLGDMG-----AEQAHREVGAYA-AGKVSALYAVGPLMAHAVQAFGA-----TGRHFA--D-Q---ASLIGALAT-EDPTTTILIKGSRSAAMDKVVAALC----

General information: TITO was launched using: RESULT: Template: 4CVK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2362 3291 1.39 7.87 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 1.39 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4CVK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CVK-query.scw PDB file : Tito_Scwrl_4CVK.pdb: