Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTEIVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL 1P27 Chain:D ((87-107)) -------------------------------------------EDIHDKFAEYGEIKNIHLNLD-----------------------------------

General information: TITO was launched using: RESULT: Template: 1P27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 14 -6332 -452.29 -301.52 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain D : 0.55 3D Compatibility (PKB) : -452.29 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_1P27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P27-query.scw PDB file : Tito_Scwrl_1P27.pdb: