Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRKIVVALGGNAILSSDPSAK--AQQEALVETAKHLVKLIKNGDDLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDEGIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAK-AEAEKSGATFKEDAGRGWRKVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFASQRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPKVEAAIAFVNGRPEGK-AVITSLENLGALIESESGTIIEKG 4JZ8 Chain:D ((4-316)) -AGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAA-AGVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYTEQEAKDLMAANPGKILREDAGRGWRVVVPSPRPLEIVEYGVIKTLIDNNVLVICTNGGGIPCKRENK-VISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACIN-----ERKLEEIKLSEILALEKDGHFAAGSMGPKVRAAIEFT--QATGKMSIITSLSTAVDALNGKCGTRIIK-

General information: TITO was launched using: RESULT: Template: 4JZ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1715 25624 14.94 84.29 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : 14.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_4JZ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JZ8-query.scw PDB file : Tito_Scwrl_4JZ8.pdb: