Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMLALISPAKTLDYETALPTDEFTQPRLLE-HSAQLIDVCRK---LSASEIASLMSVSEKIATLNADRFRDWKPEFDFSNARQAIYAFKGDVYTGLDAYHLKDKDIDFAQQHLRMLSGLYGLLRPLDLMMPYRLEMGTKLKNTHGHNLYEFWGDIITNQINEDLAAIKSELLVNLASDEYYKSVNEKKIKAEIVKPVFLDQKNGKYKVISFYAKKARGLMARFIVENQLNKVEDIKAFNTEGYYFDADNSSAKELVFKRDEQ 3EYI Chain:B ((4-38)) -------------------------SPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EYI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 52 -3725 -71.63 -120.15 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -71.63 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_3EYI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EYI-query.scw PDB file : Tito_Scwrl_3EYI.pdb: