Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MFGKILNKFKAWYK-GDPGDMRWDPRTDTYVGTREPSKHWTAKVLSYFVDFSLLIAKSIKKHPSAYITQLLAFIAILV--SCFSIYLQYYVDDDEYKRCTIAHTNNQEIALKCKK---- 5U35 Chain:A ((1-124)) GSHMTEEEVRKIMEKLKKAFKQGNPEQIVSLLSPDVKVDVGNQSFSGSEEAEKAARKLMKFVDRVEVRDVRVFENAVMIAVEFEVNGQRYKMIFTFYVENGKVSMVSIYIS--PTMKKLMKQILNY

General information: TITO was launched using: RESULT: Template: 5U35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 457 -2811 -6.15 -25.55 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -6.15 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_5U35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U35-query.scw PDB file : Tito_Scwrl_5U35.pdb: