Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNTLAERLRYAMEVLPPKKIKGVELARAVGVKP-PSVSDWLSGKSKTMEGENLLRASKFLNV-----NPSWLAS------GTGEIQTSTKDKFKQLDIEEFKKKYNISDSDEALLFSTIIEKPFIPSSKRWVPVKAYSKMGMDGYFTDIGYEGNAGDGYVPTHTAGSRAYAVKGTGDSMFPAIRNGWYVVC-DPDADLVPNEFVQVCLKDGR-CTIKEFVGINGGVLSLLSVNGGERFF--------FEMDEVESITAITDI--------------VPPSQHRQEHPYSH-- 4PHR Chain:A ((1-277)) SEFELMKRLSEIKVLPILESLKYIKHNHASVVRFGDGEIDLMTGHSIPYQDYNEKLAKRLQQILQTKSDEKLLVCLPDVFSNMDRYNQNARHFWERHFLK--YSEFYLNCCDAPFYGSTFISRPYIDL---------IDKSPSEAYFESLK-ELWRGKDLLIVEGATSRS----GVGNDLFVAASSIKRLVCPSKNAFQYYDEILRLTEKNAKNRLILVMLGPTAKVLVADLTTKGYQAIDLGHIDSEYEWYEMGATYKVKLTNKHTAEFNYDEGIELEFSQEYQEQIVARIG

General information: TITO was launched using: RESULT: Template: 4PHR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 49768 40.01 209.11 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 40.01 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_4PHR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PHR-query.scw PDB file : Tito_Scwrl_4PHR.pdb: