Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKLCDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMY-GTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQG-KDLQQVLADLKTLLTDNGFVVDYVEARQPNLLAASQFD-RDIVLFVAAKLGGTRLIDNLQVAFTPQ 3Q10 Chain:C ((21-280)) -----------------RQEGKRIALVPTMGNLHEGHMTLVDEAKTRADVVVVTIFVNPLQFERPDDLAHYPRTLQEDCEKLTRHGADLVFAPAAADIYPAGLEKQTYVDVPALSTILEGASRPGHFRGVSTIVSKLFNLIQPDVACFGEKDYQQLALIRKMVADMGYDINIVGVPTVRAKDGLALSSRNGYLTEEERQIAPQLSKIMWALAEKMALGERQIDALLEEAAAQLLRVGFTPDELFIRDAETLQPLTVDSQQAVILMAAWLGKARLIDN--------

General information: TITO was launched using: RESULT: Template: 3Q10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1198 -61059 -50.97 -237.58 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -50.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.652

(partial model without unconserved sides chains): PDB file : Tito_3Q10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q10-query.scw PDB file : Tito_Scwrl_3Q10.pdb: