Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSND----NDAYVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 5IV9 Chain:B ((1-148)) -----------------------GWHLRSTT-KVP-TTMKTMILQSSDPN-GPLSRAVRNQLRLNGVDLIDASTLRKDVPSLRLDGSSIQKDTASVFQDGRTAEYQMVMTVHASVLIP-GHDIY-PITTKVYRSFFDNPQAALAKDAEQDMIIQEMYDKAAEQLIRKLPSVQVADV--

General information: TITO was launched using: RESULT: Template: 5IV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 492 20578 41.82 148.04 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 41.82 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_5IV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IV9-query.scw PDB file : Tito_Scwrl_5IV9.pdb: