Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MMKLNALLCSAVLATSMVTVAHADNTTHVAAASALGSIAG---TAIGKSMGGTNGGYIGAALGAAGGSALGNKISKDRDADRSSKYWKKNTVIIVTNYRDEIGLKTPSGVFL 2L3N Chain:A ((1-104)) SVSILRSSVNHREVDEAIDNILRYTNSTEQQFLEAMESTGGRVRIAIAKLLSKQTSG-------GSGGSKLGGSGGSRKDLSVKGMLYDSDSQQILNRLRERVSGSTAQSA--

General information: TITO was launched using: RESULT: Template: 2L3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 209 -4811 -23.02 -48.11 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -23.02 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_2L3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L3N-query.scw PDB file : Tito_Scwrl_2L3N.pdb: