Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTALDQLKTLTTVVADSSDLEAIRKFRPLDATTNPSLITAAAEQPESKELIEDAYYQAKEEG--YKNDELI-ERTIDILTVKFGVEILKLIEGRVSTEVDTALSYDTEATIQKAHELCELYKGYGIDQSRILIKIASTWEGIQAAKVLEAEGIACNLTLLFGLHQAQACADAKVTLISPFVGRILDWYKKAEGVDSYP-IEKDPGVVSVKKIYTYYKQQNIPTQVMGASFRSIDQVLGLAGCDLLTISPSLLTQLEQDTRTVDAALDANKAKQAEAIVRPAQDEQSFKDELNHDLMAFQLLQGGVDGFIKARDQLSLLLRQSFGIDAEIKS 3TKF Chain:A ((26-345)) -QKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAETISKVKANNPDLNSDDLVKEIAIEIL-VSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIMIAATWEGIKAAKLLQKEGINCNLTLIFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKLYKSHGFKTIVMGASFRNVEQVIALAGCDALTISPVLLEEL----KNRDEHLEVKLT---------QISEADFRWLMNENAMATHKLAEGIRLFTKDTIELENIIKQNL--------

General information: TITO was launched using: RESULT: Template: 3TKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1725 -26187 -15.18 -85.58 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -15.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_3TKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TKF-query.scw PDB file : Tito_Scwrl_3TKF.pdb: