Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MNKPLSSRFLFKSFGMVAVC----MSLAACGDKAW---WSNNDEPELESQQ----IKRLIPSRVHDRESWAKDIDDIM--KDLDIPKTKQNVCSIVAVVDQESNFVANPQVPGLGQKAVEEVS-----TRLNEKFEDKLGKTIGGTIAGYFE-------EVLRTQPSPDNNYMSQMRKVKTEKDLDLLYREIFDFMAKHYHVSALTGAAKLVGQDIGEKMNPITTLGSM-----QVHINYAKA-NKRSSMNTAALRDDLYTEYGGLYYGIHR-LMVYPADYDKAIYRFADYNSGMYSSR-NAAFQKMLKELTDKDISLDGDLLLYTKDGDPRATQSESE--KELIT-----VFASNNVLVTP-------RQIRDDLKLEKEKKFESTQTY---IALTKL----------YKSKTGKEPLYAIMPQVVI----SGPKLSRDYNTNWYATRVNGR----YETCMQRAKRIRL------- 3C2W Chain:A ((5-494)) TPVTLANCEDEPIHVPGAIQPHGALVTLRADGMVLAASENIQALLGFVASPGSYLTQEQVGPEVLRMLEEGLTGNGPWSNSVETRIGEHLFDVIGHSYKEVFYLEFEIRTADTLSITSFTLNAQRIIAQVQLHNDTASLLSNVTDELRRMTGYDRVMAYRFRHDDSGEVVAESRREDLE--SYLGQRYPASDIPAQARRLYIQNPIRLIADVAYTPMRVFPALNPETNESFDLSYSVLRSVSPIHCEYLTNMGVRASMSISIVVGG---KLWGLFSCHHMSPKLIPYPVRMSFQIFSQVCSAIVERLEQGRIAELLRVSTERRLALARRA--RDADDLFGALAHPDDGIAALIPCDGALVMLGGRTLSIRGDFERQAGNVLQRLQRDPERDIYHTDNWCCGVLAIRFHRQESGWIFWFRHEEVHRIRWGGKPEKLLTIGPSGPRLTPRGSFEAWEEVVRGHSTPWSETDLAIAEKLRLDLMELCL

General information: TITO was launched using: RESULT: Template: 3C2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1787 24716 13.83 62.57 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.83 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_3C2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C2W-query.scw PDB file : Tito_Scwrl_3C2W.pdb: