Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNFARQHFRKHQA-KSAAEQAAEFGSMKNAT---------AYELQLMQLNNDRARL--------KQIQSTENK------IKLKAELLPTYAPYIDGILEA--QSGVQDEILTEIMIWNIDTSNFSRALQI---AEYVLVHNLALPDRFERTPACVITEEISAAFLKQLKTNVEI---DIDVLKQLESLM--TNPDLPEKTLD--MPDQVKAKM----YLALGKAEI-------RFISDK----DKPDLVHTKAAADYLQKAVELDDKCGGRGDLNMVQKLLDKFAPATEANKGDAAASS----------- 4U9O Chain:A ((14-332)) PIAGTPSQVISDGKAIKKVALLGEEYVGMRPTMHVRVGDEVKKAQILFEDKKNPGVKFTSPVSGKVVEINRGAKRVLQSVVIEVAGDDQVTFDKFEANQLASLNRDAIKTQLVESGLWTAFRTRPFSKVPAIDSTSEAIFVTAMD-TNPLAAEPTVVINEQ-SEAFVAGLDVLSALTTGKVYVCKKGTSLPRSQQPNVEEHVFDGPHPDHVAWSINYQDVIAVGQLFLTGELYTQRVVSLAGPVVNKPRLVRTVMGA-SLEQLVDSEIMPGE------VRIISGSVLSGTKATGPHAYLGRYHLQVSVLREG

General information: TITO was launched using: RESULT: Template: 4U9O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1219 53302 43.73 223.02 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 43.73 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_4U9O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U9O-query.scw PDB file : Tito_Scwrl_4U9O.pdb: