Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQVYLVGGAVRDYLLGHS---YQEKDYVVVGATPEHMLAQGFQPVGKDFPVFLHPETKEEYA-LARTERKSGQGYHGFQFFTDT------TVSLEDDLIRRDLTINAIAMDQD---------GKIYDPYGGQNDLENKILRHVSE-AFAEDPLRVLRVARFAARYFPYGFQIAPETLQLMQTMADSGELDALTPERVWKETSRALMENHADIYFQTLRDCGALKHLFPEIDALFGVPQRPEYHPEVDCGIHTLMSLQQACKS-------------------------NYSLDVRFAVLVHDLGKALTPAKELPRHIMHEERGIKPVTQLCERLRVPTQTKQLALSVCKEHLKCHQIMS------LKPGTLWRLLQRLDVLRRPERVEAFVQACECDAKGRLGLEDRPYPQAQYMREAMQIVRSIKVQDLPENIKGAEIGEMLIQYRIEALAEFKNQHQSLSHS 3WFP Chain:C ((21-411)) -YCFIVGGWVRDRILGEPVGYNIDVDFLTT-ADPVELAKNFAKRIGGHFFVFEKRGFLIKRPTIASVVL-HLPPY-RYRFDFSPLKGKDLEKALIEDLKERDFTANAIAVNLDDVLSIGAKQTIVYDPTGGIKDLEQGLLRPVSIENLKRDPVRVLRGFRIAIEK---NLQLTEDFYEFVKEDP--RIVLKSAVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKD--------PLDEHTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGALFHDIGKPQTF----VTFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHLRPFFLREAFKKGELKRRGMANFWRECGD-----IAPHLFLLSIADAMASGDEE-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1517 5465 3.60 16.71 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 3.60 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3WFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFP-query.scw PDB file : Tito_Scwrl_3WFP.pdb: