Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATNYGTGRRKTATARVFLSAGTGKLVINNRTLEQYFGRETARMVVRQPLELLEATEKYDLYITVKGGGIGGQAGAIRHGITRALIAADETLKPVLRQAGFVTRDAREVERKKLGLRKARKRPQFSKR 5O5J Chain:I ((25-150)) --PIQTVGRRKEAVVRVRLVPGTGQFNLDGRTLENYFPNKVHQQLIKAPLVTVDRVDQFDIYAHLDGGGPSGQAGALRLAIARALILVQPEDRPALKKAGFLTRDPRAIERKKYGLKKARKAPQYSKR

General information: TITO was launched using: RESULT: Template: 5O5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 505 -56433 -111.75 -447.88 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain I : 0.84 3D Compatibility (PKB) : -111.75 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_5O5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5O5J-query.scw PDB file : Tito_Scwrl_5O5J.pdb: