Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEEKMNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALKEADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQNQG 2E2A Chain:A ((2-104)) ------NREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVVEAGSCIAEAHSSQTGMLAREASGEELPYSVTMMHGQLHLMTTILLKDVIHHLIELYKRGA-

General information: TITO was launched using: RESULT: Template: 2E2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 344 -30349 -88.22 -294.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -88.22 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_2E2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E2A-query.scw PDB file : Tito_Scwrl_2E2A.pdb: