Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASLRQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLPKKVRRLAMKSVLSEKVAENNLVAIEGLN-FDAPKTKEFKQVLANLSIDSKVLVVLEKGNDFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA 5O60 Chain:E ((2-209)) TLKVDVKTPAGKTDGSVELPAELFDVEPNIALMHQVVTAQLAAKRQGTHSTKTRGEVSGGGKKPYRQKGTGRARQGSTRAPQFTGGGTVHGPKPRDYSQRTPKKMIAAALRGALSDRARNDRIHAVTELVEGQTPSTKSAKTFLGTLTENKKVLVVIGRTDEVGAKSVRNLPGVHVISPDQLNTYDVLNADDVVFSVEALNAYISANS

General information: TITO was launched using: RESULT: Template: 5O60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 846 -21114 -24.96 -102.00 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain E : 0.80 3D Compatibility (PKB) : -24.96 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.644

(partial model without unconserved sides chains): PDB file : Tito_5O60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5O60-query.scw PDB file : Tito_Scwrl_5O60.pdb: