Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEKGVETMKIAVDAMGGDHAPQAIVEGVALAQQDF-PEVEFLLYGKEAEIKKYLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTG-ALLAAGLFIVGRI---KNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTEATK---GSEVTKKAYELLQNETSL-NFIGNVEARE--------------LLNGVADVVVT-DGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 1YCO Chain:A ((1-275)) -------MITVSIAGGSQ----PEILQLVKKALKEAEQPLQFIVFDTNENLDT----ENLWKYVHCSD---------------EAAVAQEAVSLVATGQAQILLKGIIQTHTLLKEMLKSEHQLKNKPILSHVAMV-ELPA--G--KTFLLTDCAMNIAPTQATLIEIVENAKEVAQK-LGLHHPKIALLSAAENFNPKMPSSVLAKEVTAHFNDQ-QEATVFGPLSLDLATSEEAVAHKRYSGPIMGDADILVVPTIDVGNCLYKSLTL------------FG-------------------------HAKV-GGTIVGTKVPVVLTSRSDSTESKFHSLRFAMRQVHH---------------

General information: TITO was launched using: RESULT: Template: 1YCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1310 -28947 -22.10 -115.32 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -22.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_1YCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YCO-query.scw PDB file : Tito_Scwrl_1YCO.pdb: