Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAIN--GYGEAQD-VFEEIDAHPR-EVGSGHVRVEIKAFSVNPYDVALRLGEMKEI--RTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHA----------------------------VGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLT--EIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASS-KNEEKVKKLGASAFAAYDKTNP----------------------GL---QF---ADQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDLD--LRQ---KKEGFYE-SFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 4GI2 Chain:A ((35-411)) MYAWAIRRERHGPP-EQSHQLEVL-PVWEIGDDEVLVYVMAAGVNYNGVWAGLGEPISPFDVHKGEYHIAGSDASGIVWKVGAKVKRWKVGDEVIVHCNQDDGDDEECNGGDPMFSPTQRIWGYETGDGSFAQFCRVQSRQLMARPKHLTWEEAACYTLTLATAYRMLFGHAPHTVRPGQNVLIWGASGGLGVFGVQLCAASGANAIAVISDESKRDYVMSLGAKGVINRKDFDCWGQLPTVNSPEYNTWLKEARKFGKAIWDITGKGNDVDIVFEHPGEAT-FPVSTLVAKRGGMIVFCAGTTGFNITFDARYVWMRQKRIQGS-H---FAHLKQASAANQFVMDRRVDPCMSEVF--PWDKIPAAHTKMWKNQHPPGNMAVLVN-

General information: TITO was launched using: RESULT: Template: 4GI2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1573 -4164 -2.65 -13.65 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -2.65 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4GI2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GI2-query.scw PDB file : Tito_Scwrl_4GI2.pdb: