Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------MAKLI-VLGGLIYGGYWLYKYMLAHP--------NIDKGKVIKT-VGV---IIVGLIALTKKNNTNSPKIMRTYRC-RGCGREISTSFNPSESPQGRDRCHTGYRHYWERID----- 4LQK Chain:A ((4-135)) SFKLILAEYIRHRNTISGNIYSALMTLDDLAIKQYGDIDLLFNEKLKVDSDSGLFDFVNFVKDMICCDSRIVVALSSLVSKHWELTNK---KYRCMA-LAEHISDSIPISELSRLRYNLSKYLRGHTESIEDKFDY

General information: TITO was launched using: RESULT: Template: 4LQK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 312 10867 34.83 115.60 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 34.83 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.087

(partial model without unconserved sides chains): PDB file : Tito_4LQK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LQK-query.scw PDB file : Tito_Scwrl_4LQK.pdb: