Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGFNLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEK-KVVLAG------GKKLDFDRLILATGSNQKFRNISA---ESTLIHPVKKIQNVTALQQKI--MNAKKIAIIGAGQAGIEIAEGLASRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENET-AIVETEKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVTFRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEEESIFYPQKIETGYVDY--KLTAIN-QSKVKLKFLFDSE-KKILGAQLQSKKEMFHLLDQLTIAVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED 1NHP Chain:A ((1-430)) --MKVIVLGSSHGGYEAVEELLNLHPDAEIQWYEKGDFISFLSAGMQLYLEGKVKDVNSVRYM-TGEKMESRGVNVFSNTEITAIQPKEHQVTVKDLVSGEERVENYDKLIISPGAVPFELDIPGKDLDN--IYLMRGRQWAIKLKQKTVDPEVNNVVVIGSGYIGIEAAEAFAKAGKKVTVIDILDRPLGVYLDKEFTDVLTEEMEANNITIATGETVERYEGDGRVQKVVTDKNAYDADLVVVAVGVRPNTAWLKGTLELHPNGLIKTDEYMRTSEPDVFAVGDATLIKYNPADTEVNIALATNARKQGRFAVKNLEEPVKPFPGVQGSSGLAVFDYKFASTGINEVMAQKLGKETKAVTVVEDYLMDFNPDKQKAWFKLVYDPETTQILGAQLMSKADLTANINAISLAIQAKMTIEDLAYADFFFQPAFDK---------------

General information: TITO was launched using: RESULT: Template: 1NHP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2383 45327 19.02 109.75 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 19.02 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_1NHP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NHP-query.scw PDB file : Tito_Scwrl_1NHP.pdb: