Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNP--ERIAVAAVDQDELVGFIGAIPQYG----ITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNAN-----GWDKPDIWMAKTIIPRPNSQ 4XNH Chain:C ((7-175)) -TLDNVY---ANNLGMLTKLAHVTVPNLYQDA---FFSALFAEKDVHFTQMAYYSEIPVGGLVAKLVPKNELSLKGIQIEFLGVLPNYRHKSIGSKLLKFAEDKCSECHQHNVFVYLPAV-------------------------DDLTKQWFIAHGFEQVGETVNNFIKGVNGDEQDAILLKKHI-------

General information: TITO was launched using: RESULT: Template: 4XNH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 563 -8568 -15.22 -59.91 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -15.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_4XNH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XNH-query.scw PDB file : Tito_Scwrl_4XNH.pdb: