Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMIKDIRFWVCVIGIVILGFLSGLLS-GNPGKYYYSLQLPPFAPPSWIFGPMWTLLYILMGISLYLLLNHNNK--KQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN 2N02 Chain:A ((5-158)) -----WVPAVGLTLVPSLGGFMGAYFVRGEGLRWYAGLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFTTVLNYYVWRDN

General information: TITO was launched using: RESULT: Template: 2N02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 552 -85932 -155.67 -569.09 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -155.67 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_2N02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N02-query.scw PDB file : Tito_Scwrl_2N02.pdb: