Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQERLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQD--FAKVMDKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKLSEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGA-NVLDD--EGEVTIDTEKMREALSFYQNLSQYT-M-PGS--NDVTEIKDAFMNGTAPMAIYSTYILPSVYEAG---NSENIGFA-IPTQKDQ---A--VYG-TVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKWVLMSPGGAQPVNKQVVELDGYKENEVIK-SFGELPSEIASAFDEVQVFGLVGEKNFTKMGDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK 4G68 Chain:C ((39-430)) -------------------------------ITLTFWNLFTGEPAKTKVKEIIDQWNKENPNVQIVESVTENDAYKTKIKAAIAANEAPDIFQTWAGGFSQPFVEAGKVLQLDSYLN---DGTKDQLLPGSFDNV-TYNGKIY-GIPFDQQASVLYINKELFDKYNVKVPTTFSELIDAIKTFKSKG--VTPFALGEKDEWPGMWYYDMIALREGGVQLTRDALNGKASFDNQAFTDAAQKLQDMVNAGAFDSGFMGLTRDEATAEFNQGKAAMYFGGNFDAAAFVSDPSSLVKGKIEAVRFPTIEGGKGDPTEYIGGTVGALMVSAN--SKYKDEAVRAAKYLAKQLSDMDYLI--AT-GLPAWKYDNIDQSK-VDPLEIQIMNN----IVANAKG--SVPAWDIYLSG---DA--AQTHKDLVAQLFAKQITPEEYSKQMQQKIN

General information: TITO was launched using: RESULT: Template: 4G68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2135 92402 43.28 248.39 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : 43.28 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4G68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G68-query.scw PDB file : Tito_Scwrl_4G68.pdb: